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The three-pool and first-order model separates the mineralizable organic carbon into active, slow, and passive carbon pools. This paper used the model and decomposition curves of the soil organic carbon to fit the active pool and its decomposition rate, slow pool and its decomposition rate. The results showed that the size of the active pool from different profiles accounted for 2.09%-3.08% of the...
The nuclear receptor peroxisome proliferator-activated receptor γ (PPAR-γ) is a key transcriptional regulator of adipocyte differentiation. It also modulates the synthesis of adipocytokines in the adipose tissue. Its polymorphisms are associated with the risk of type II diabetes, obesity, cardiovascular diseases and cancer. In the present study, to investigate the regulatory mechanism of PPAR-γ gene...
The gene encoding nonstructural protein N pro of BVDV was amplified by PCR, then was inserted into pEGFP-C1 vector. The 293 cells were transfected in vitro with recombinant plasmid by liposome. N pro - EGFP fusion protein was viewed directly with fluorensce microscope and mRNA expression of N pro was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results...
Response surface methodology was applied to optimize medium components for production of recombinant calf chymosin by Kluyveromyces lactis GG799. The previous data indicated that the most suitable carbon source, nitrogen source, salt and vitamin were glucose, yeast extract, KH 2 PO 4 and Ca D-Pantothenate, respectively. The concentration of four media components were optimized by using...
A detecting method based on machine vision was put forward to test the performance of seedmeter with corn and soybean seeds as test samples, in which MATLAB software was applied to process image data and analyze the results. The experimental results showed that the mean value of absolute error of the sowing speed for soybean was 0.004-0.68 seed · s −1 ; the mean value of relative error was...
With the startup and execution of new socialistic countryside construction in Heilongjiang Province, the transition from castoff to resource is strengthened in the countryside, aiming at neat appearance of the countryside, clean production and saving energy. People produce biogas and provide the countryside with new energy by means of turning livestock's dejection into resources, composting of the...
Heilongjiang is a large agriculture province. Problems of agriculture, rural areas and farmers are urgent to be solved. The development of agriculture needs the support of agricultural credits, because finance is the center of agriculture economy. However, the low comparative advantage in agriculture and pursuit of the capital interests which aggravate the conflicts of supply and demand of agricultural...
Antimicrobial peptides are widely distributed in nature, existing in organisms of plants, insects, and vertebrates. It has been approved that antimicrobial peptides have broad spectrum antimicrobial activities, and play a key modulatory role in the innate immune response and tumor inhibiting activity. Due to the special action mechanism, the antimicrobial peptides become a hot field of genetic engineering...
Nitrogen (N) is considered as an important yield and quality-determining factor in soybean production. The objective of the current experiment was to determine the regularity of N accumulation and its simulation among different varieties. This work was conducted with frame tests (pot without bottom), and three cultivars, HH41, SN14, HN40, and one feed soybean variety MSD was used as materials. The...
The regulating axillary branch gene was cloned and named as CsCCD7. Using a series bioinformatic computer softwares, database and online programes, CsCCD7 nucleotide sequence and CsCCD7 amino acid sequence were analyzed and CsCCD7 function was predicted. The results showed that CsCCD7 cDNA full length sequence was 2 136 bp, and included a 1 665 bp ORF which encoded a 554 AA protein; there were 32...
In order to optimize polymerase chain reaction (PCR) amplification of the internal transcribed spacer (ITS) rDNA region from fungi found in black soil in North China, an orthogonal experimental design [L 16 (4 5 )] was used to evaluate five factors (template, Mg 2+ , dNTP, Taq DNA polymerase, and primer) from four levels. Subsequently, the optimal annealing temperature, annealing...
The feasibility of molecule markers' application in gene pyramiding has been proved, and obvious progresses in crop breeding have been made till now. Furthermore, different QTLs or molecular markers linked tightly to yield, quality or resistance may be used for marker assisted selection. MAS will be applied widely in crop breeding due to the development of more gene-based markers and efficient quantitative...
In this study, a rapid molecular marking method, gspCAPS (genome sequence pool CAPS), was established to develop soybean CAPS markers. The bioinformatics tools were executed for analyzing the sequences of soybean SNP sites and selecting the appropriate restriction endonucleases. Meanwhile, DNA samples were mixed, and the primers were sequentially screened by PCR and enzyme digestion procedures. To...
The effects of the microspore developmental stage, hormones and culture condition on anther in vitro culture of lily (Lilium spp.) were discussed. The results showed that when the flower buds were about 23-26 mm long, the microspores were at the uninucleate stage which was suitable for culture and the culture under the darkness would promote the callus induction of anther. The induction frequency...
Rural territorial function follows the trend to develop from agricultural production space to a complex of cultural heritage, food security, social stability, quality of living, etc. Based on the rural area's territorial function to both urban and rural areas and its evolution law, index system of rural development evaluation, covering rural production function, rural consumption function, regional...
In order to enhance the seed storability and supply high-quality seeds for vegetation restoration in the arid and semiarid regions in Northwest China, the effects of ultra-drying and accelerated aging on the physiological characterstics of Melilotus suaveolens seeds were studied. Melilotus suaveolens seeds were dried in a desiccator containing silica gel to 80, 53, 42, 33, 23 and 16 g • kg −1...
The traditional technique for asexual propagation of Siraitia grosvenori in vitro is adopted widely by pressing the vine, which has a high risk of carrying viral diseases and limits the production of promoted cultivars. So this paper reported in vitro regeneration of S. grosvenori by testing for shoot induction from various explant sources such as leaf, petiole and stem. Several phytohormone combinations...
By two years (2007–2008) located fertilizer experiment, the effect of long-term combined application of organic and inorganic fertilizers on black soil fertility and crop yield was investigated in Shuangcheng City, Heilongjiang Province. The results showed that the combined application of organic and inorganic fertilizers could increase the organinc matter, alkaline nitrogen, available phosphorus...
Blueberry is a kind of wild berry with high nutrition values. It contains a great amount of anthocyanin, organic acid and fiber, which significantly affects sensory evaluation, pH value, texture profile analysis (TPA) and microstructure. This study determined the effect of blueberry pulp on spreadable processed cheese through examining pH value, TPA, microstructure and color-difference. The results...
Dongnong50 was overexpressing TaDREB3, a stress-tolerance gene, and transgenic progenies with TaDREB3 showing stable heredity characters were obtained in this study. The seeds of T 4 generation were sowed on saline land of which salt concentration was 0.19% and pH was 8.43, and agronomic traits including growth period, plant height, numbers of main nodes, branching numbers, pods per plant,...
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